OASL Expression in Pancreatic Cancer and Its Effect on Proliferation and Migration of Pancreatic Cancer Cells / 肿瘤防治研究

Objective To explore the effect of OASL expression on the proliferation and migration of pancreatic cancer cells. Methods The GEPIA database was used to analyze the differences in OASL expression in pancreatic cancer tissues and normal pancreatic tissues. The TIMER database was used to analyze the relationship between OASL expression and patient survival. The TCGA database was used to analyze the correlation of OASL expression with the clinicopathological parameters of pancreatic cancer. shRNA was used to knock down the expression of OASL gene in pancreatic cancer panc-1 cells. Lentiviruses were used to overexpress the OASL gene in pancreatic cancer cells. MTT assay was used to evaluate their proliferation ability, and scratch and Transwell experiments were used to evaluate their migration ability. Western blot experiments were used to detect changes in proteins related to tumor proliferation, migration, and invasion. Results OASL expression in the pancreatic cancer group was significantly higher than that in normal pancreatic tissue (P < 0.05), and patients with high OASL expression in pancreatic cancer patients had worse OS than patients with low expression (P < 0.05). After OASL gene knockdown, the proliferation and migration abilities of panc-1 cells were inhibited, whereas the overexpression of OASL gene promoted the proliferation and migration ability of panc-1 cells. Western blot experiments showed that after OASL knockdown, p-STAT3 protein expression increased, whereas STAT3 and BAK protein expressions decreased. After OASL overexpression, p-STAT3 protein expression decreased, and STAT3 and BAK protein expression increased. Conclusion OASL may affect the proliferation and migration of pancreatic cancer cells through the STAT3 signaling pathway while affecting BAK expression to induce cell death.

Debridement， Antibiotics and Implant Retention for the Treatment of Periprosthetic Joint Infection After Arthroplasty： A Narrative Review / 中山大学学报(医学科学版)

Periprosthetic joint infection （PJI） following joint arthroplasty is devastating and technique-demanding. At present， the surgical treatment for PJI includes debridement， antibiotics， and implant retention （DAIR）， single- or two-stage revision， arthrodesis， and amputation. DAIR is appealing to both surgeons and patients as it can avoid unnecessary implants removal， making it less time-consuming and less invasive. In this article， we review the current knowledge in surgical timing， intraoperative details and antibiotics strategy of DAIR.

Exploration and practice of experimental skills training for young teachers of basic medical science / 中华医学教育探索杂志

With basic medicine teaching faculty becoming younger,it is still in accordance with the traditional training mode about training the young teachers' experimental skills, which brings about some problems such as nonstandard basic operation, weak basic knowledge, lack of systematic skills training and relative lack of research.In practice,we strengthen the basic skills of young teachers through skills training and assessment, focus on the cultivation of young teachers' basic quality by paying attention to theoretical teaching skills and experimental skills; guide teachers to emphasize experimental skills through holding ex-perimental skills competition; promote teachers' experimental skills by integrating the subject research into experiment practice which proved that the experimental skills and teaching level of young teachers get sig-nificantly improved.